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KMID : 0364820120480010048
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Korean Journal of Microbiology
2012 Volume.48 No. 1 p.48 ~ p.51
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Production Properties on Extracellular Protease from Chryseobacterium Novel Strain JK1
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Lee Yu-Kyong
Oh Yong-Sik
Roh Dong-Hyun
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Abstract
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A novel Chryseobacterium sp. JK1 strain producing extracellular protease had been isolated from soil. The largest
clear zones were observed on nutrient agar plates supplemented with 1% skim milk at 30?35℃ along with the growth
of Chryseobacterium sp. JK1. The cell growth of JK1 strain was maximal at 24 h and maximum protease activity
was reached up to 560 unit/ml at the stationary phase in liquid culture. In the presence of maltose, glucose or
mannitol in Nutrient broth, cells grew well, but protease were produced poorly with lower production yields of
64?77% than in NB broth only. Similarly, the addition of skim milk, beef extract, yeast extract, malt extract or
tryptone showed good growth and poor enzyme production. On the contrary, the addition of (NH4)2HPO4 or
(NH4)2SO4 gave poor growth and good enzyme production of 121?146%.
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KEYWORD
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Chryseobacterium sp. JK1, production condition, protease
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